Effects of exercise and nutrition on skeletal muscle

Project Number
RI 12/12 YYF

Project Duration
April 2013 - September 2015

Status
Completed

Abstract
The purpose of this study is to investigate the effects of exercise and nutrition on skeletal muscle. Exercise and nutrition are both integral to the prevention, treatment and management of many chronic health conditions and skeletal muscle plays a pivotal role in quality of life, long-term health, and survival outcome in various chronic health conditions. In order to better understand the effects of various exercise and nutrition interventions, one needs to study the molecular responses and adaptations to these interventions. As such, the specific aim of this study is to characterise the global gene expression in skeletal muscle of young men after an acute bout of unilateral resistance exercise (RE) in a fed state. A total of 8 young (20-30 y) healthy and recreationally active men will be recruited to perform 3 sets of 10 repetitions of unilateral knee extensions at 70-75% of their concentric one-repetition maximum. Following the RE, participants will consume a protein drink containin g 20 g of protein. A total of three muscle biopsies from the vastus lateralis will be obtained: one from either of the legs before RE and one each from the exercised and control legs at 4 hours post-RE. The global gene expression in the vastus lateralis at the two time points will be quantified using microarrays. This will be the first study to characterise the global gene expression in human skeletal muscle following an acute bout of RE in a fed state as opposed to a fasted state. In addition, this is the first study outside of the United States of America and Europe to quantify global gene expression after an acute bout of RE. The results from this study will serve as a baseline comparison for future studies investigating changes in global gene expression in relation to sex, age, training, race and health conditions following either exercise or nutrition, or both interventions. In turn, one can then better understand the responses and adaptions to exercise and nutrition interventions for a healthier living, and the prevention and management of various chronic health conditions. This study bridges the gap between exercise, nutrition and medical sciences and its objectives are aligned with Singapore's push for healthy lifestyle through regular physical activity and healthy diet. for a healthier living, and the prevention and management of various chronic health conditions. This study bridges the gap between exercise, nutrition and medical sciences and its objectives are aligned with Singapore's push for healthy lifestyle through regular physical activity and healthy diet.

Funding Source
NIE

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