Facile Formulation for "Moisturiser" for Plants

Project Number
NTU-MSE-Facile Formu

Project Duration
November 2015 - October 2018


The water retention capability will be evaluated in plants. Three different plant growth conditions are proposed: (1) Aseptic plant tissue culture condition; (2) Greenhouse with manual irrigation; (3) Growth chamber with artificial drought and heat simulation; (4) Field condition. In aseptic square petri dishes the widely studied model plant Arabidopsis thaliana will be grown in half MS medium. The petri dishes will be placed vertically and be sealed with micropore surgical tapes. Throughout the plant growth, transpiration water will go out of the petri dish through the tape but no water will be supplemented into the dish. It's an ideal system to test water retention capability of the candidate nanomaterials. Any water deficit would lead to the change of plant root architecture (root length, surface area, number of lateral roots, and root hairs). Additionally we can use this model plant to assess any adverse or toxic effects brought by the nanomaterials. Potted plants placed in greenhouse @ National Institute of Education, NTU will be irrigated manually. Other than the irrigation water the ambient humidity contributes to the water availability to plants as well, thus we propose to use plant growth environmental chamber to simulate the drought and heat conditions brought by the El Nilio climate threatening vegetation in Singapore in recent years. We will also perform field investigations of plant growth and health at selected NParks sites with proper setting of control and experimental areas. In addition to the Arabidopsis thaliana used in tissue culture condition, we plan to use various plant species ranging from grass, shrubs to trees under greenhouse, growth chamber and field conditions.

Funding Source
A/P Lam Yeng Ming

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